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HuProt全人类蛋白质组芯片


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涵盖>21,000个人重组蛋白，覆盖81%的编码基因。

每个蛋白重复2点，1张芯片包含>40,000个指标。

>500篇文献报道使用，久经考验。


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最高覆盖度的人类重组蛋白质库：


        HuProtTM蛋白质组芯片的背后是一个高覆盖度的重组蛋白质库，涵盖>21,000个人重组蛋白，对应16,794个编码基因。有的基因包括多个剪切异构体（Isoform）或片段（fragment），大多数蛋白为全长蛋白。

       这些蛋白包含大部分功能性蛋白，如胞内蛋白、膜蛋白、酶、分泌蛋白、转录因子，GPCRs，细胞因子、受体分析、CD分子、离子通道、细胞溶质蛋白、核受体等。另外，多数组织特异或相关的蛋白也都涵盖，比如睾丸、大脑皮质、甲状腺、皮肤、输卵管、肝脏、甲状旁腺、肠、肾、脾、肌肉、附睾、淋巴结、骨髓、肾上腺、食道、心脏、阑尾、扁桃体、前列腺、直肠、脂肪组织、胃、结肠、宫颈、子宫、胆囊、精囊、乳腺、卵巢、子宫内膜、平滑肌、唾液腺、胰腺和膀胱。 

   


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应用领域

抗体组学研究

自身抗体谱检测

小分子靶标鉴定

蛋白质/DNA/RNA相互作用蛋白鉴定



技术文档

Huprot蛋白列表

HuProt_V4.0.zip



蛋白质芯片应用手册

HuProt_Human_Proteome_Microarray_v4_0_User_Manual_Rev230911.pdf



参考文献


Autoantibody Profiling

•Gruber C. et al (2020). Mapping Systemic Inflammation and Antibody Responses in Multisystem Inflammatory Syndrome in Children (MIS-C). Cell. 2020 Nov 12;183(4):982-995.e14.

•Li Y. et al (2020). Longitudinal serum autoantibody repertoire proflining identifies surgery-associated biomakers in lung adenocarcinomas. EBioMedicine. 2020 Mar;53:102674.  

•Pan, J. et al (2020). Integration of IgA and IgG Autoantigens Improves Performance of Biomarker Panels for Early Diagnosis of Lung Cancer. Mol Cell Proteomics. 2020 Mar;19(3):490-500. 

•Osman, I. e al (2020). Using autoantibody signatures to predict immunotherapy discontinuation in melanoma patients. J. Clinical Oncology 38 (15). 

•Longobardi, S. et al (2020). Novel shared antibody specificities in anti-Ro/ La antibody negative Sjögren’s Syndrome. J Immunol May 1, 2020, 204 (1 Supplement) 218.20 

•Dhande, I. et al. (2020). Stim1 Polymorphism Disrupts Immune Signaling and Creates Renal Injury in Hypertension. Journal of the American Heart Association. 2020;9 

•Zhang, S. et al. (2020) .Autoantibody signature in hepatocellular carcinoma using seromics. Journal of Hematology & Oncology. 13 (85) 

•Zaenker, P. et al. (2020). Tropomyosin autoantibodies associated with checkpoint inhibitor myositis. Oncoimmunology. 9(1) 

•Ma, A. et al (2020). Serum Levels of Autoantibodies Against Extracellular Antigens and Neutrophil Granule Proteins Increase in Patients with COPD Compared to Non-COPD Smokers. International Journal of Chronic Obstructive Pulmonary Disease (15): 189—200.

•Kim, S. et al. (2020). B Cells Improve Overall Survival in HPV-Associated Squamous Cell Carcinomas and Are Activated by Radiation and PD-1 Blockade. Clin Cancer Res 2020;26:3345–59 

•Rowley, A. et al. (2020). A Protein Epitope Targeted by the Antibody Response to Kawasaki Disease. J. Infectious Diseases, 222(1):158–168 

•Ling. H. et al. (2020). Discovery of new serum biomarker panels for systemic lupus erythematosus diagnosis. Rheumatology Volume 59, Issue 6, June 2020, Pages 1416–1425


Protein Interactomics

•Kim, S. et al. (2020). Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 2020, 9(3), 595. 

•Lucia, C. et al. (2020). Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein. Scientific Reports 10:1609 

•Chen X. et al (2020). Celastrol induces ROS-mediated apoptosis via directly targeting peroxiredoxin-2 in gastric cancer cells. Theranostics 2020; 10(22):10290-10308.

•Chen, P. et al (2020). High-throughput screening suggests glutathione synthetase as an anti-tumor target of polydatin using human proteome chip. International Journal of Biological Macromolecules Volume 161, 15 October 2020, Pages 1230-1239. 

•Garranzo-Asensio, M. et a. (2020). Protein Microarrays: Valuable Tools for Ocular Diseases Research. Current Medicinal Chemistry, Volume 27, Number 27, 2020, pp. 4549-4566(18)